Thalidomide
Ka morao
Thalidomide e ile ea hlahisoa e le sedative, immunomodulatory agent hape e fuputsoa bakeng sa ho phekola matšoao a mefuta e mengata ea kankere. Thalidomide e thibela E3 ubiquitin ligase.,e leng setsi sa CRBN-DDB1-Cul4A.
Tlhaloso
Thalidomide qalong e khothalletsoa e le sedative, inhibits cereblon (CRBN), karolo ea cullin-4 E3 ubiquitin ligase complex CUL4-RBX1-DDB1, e nang le Kd ea∼250 nM, 'me e na le immunomodulatory, anti-inflammatory le anti-angiogenic kankere.
Ka Vitro
Thalidomide qalong e khothalletsoa hore e be sedative, e na le immunomodulatory, anti-inflammatory le anti-angiogenic cancer properties, le targets cereblon (CRBN), karolo ea cullin-4 E3 ubiquitin ligase complex CUL4-RBX1-DDB1, e nang le Kd ea∼250 nM[1]. Thalidomide (50μg / mL) e etsa hore mosebetsi oa anti-tumor oa icotinib o be khahlanong le ho ata ha lisele tsa PC9 le A549 ka bobeli, 'me phello ena e amana le apoptosis le ho falla ha lisele. Ho phaella moo, Thalidomide le icotinib li thibela litsela tsa EGFR le VEGF-R2 liseleng tsa PC9 [3].
Thalidomide (100 mg/kg, po) e thibela ho beoa ha collagen, e theola boemo ba polelo ea mRNA.α-SMA le collagen I, mme e fokotsa haholo li-cytokine tsa pro-inflammatory ho litoeba tsa RILF. Thalidomide e fokotsa RILF ka ho hatella ROS le ho theola taolo ea TGF-β/Smad tsela e itšetlehileng ka boemo ba Nrf2[2]. Thalidomide (200 mg/kg, po) e kopantsoeng le icotinib e bonts'a litlamorao tsa synergistic anti-tumor ho litoeba tse hlobotseng tse nang le lisele tsa PC9, ho hatella kholo ea hlahala le ho khothaletsa lefu la hlahala[3].
Polokelo
Phofo | -20°C | 3 lilemo |
4°C | lilemo tse 2 | |
Ka solvent | -80°C | Likhoeli tse 6 |
-20°C | 1 khoeli |
Sebopeho sa lik'hemik'hale
Tlhahiso18Merero ea Tlhahlobo ea Boleng e amohetsoeng4, le6merero e tlas'a tumello.
Sistimi e tsoetseng pele ea taolo ea boleng ba machabeng e ralile motheo o tiileng oa thekiso.
Tlhokomelo ea boleng e tsamaisana le potoloho eohle ea bophelo ba sehlahisoa ho netefatsa boleng le phello ea kalafo.
Sehlopha sa Litaba tsa Taolo ea Setsebi se tšehetsa litlhoko tsa boleng nakong ea kopo le ngoliso.